Part:BBa_K3059620

pre-csgE RBS

RBS to follow csgBAC and precede csgEFG operon in a synthetic curli operon.

The native curli operon in MG1655 E. coli contains two divergent operons: csgBAC as well as csgDEFG. To create a synthetic curli operon, both csgBAC and csgEFG are necessary (for example, though csgA is the main subunit of the eventual fiber, csgG is responsible for fiber export out of the cell). csgD regulates csgBAC, and is thus unnecessary in a synthetic operon where both component operons are combined under the control of one promoter.

When successfully assembled in a transcriptional unit with a promoter, csgBAC, csgEFG, and a terminator, this RBS results in curli amyloid fiber production. Curli fibers constitute the main proteinaceous component of Enterobacteriaceae species such as E. coli (Barnhart & Chapman, 2006).

Proposed transcriptional unit with this part. For a 3G-compatible version of this part (and more information on construction of a complete synthetic curli operon), see BBa_K3059629.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]